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Ascentis Express HILIC (silica), 4.6 X 75 mm (2.7 um). ACN:ammonium formate, 40 C 1.85 mL/min = 100 bar 3.0 mL/min = 165 bar 4.0 mL/min = 226 bar Plate counts were 12,000 to 15,000 for several analytes. I was generally please with the performance,…
1 hour ago
I have used the Imtakt amino phase, the PFP phases from Supelco and Restek (mixed mode but not well understood), and bare silica from Supelco (mixed mode in a different way). I was able to develop good separations on all phases, although it did requ…
1 hour ago
The "best" HPLC is the one that meets the needs of your laboratory - precision, accuracy, cost, reliability, etc. If you do not have a difficult separation, many instruments may be the "best." If your problem is more complex, then you may need speci…
1 hour ago
It is unfortunate that the regulatory agencies make these decisions based on a poor scientific understanding of the problem. The conductivity measures the presence of ions only in solution. As stated below, this is only important if you are perform…
2 hours ago
Merlin K. L. Bicking, Ph.D., Larry Kang, Tania Trower and 2 more joined CHROMmunity
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Tim Bannerjee

Back Pressure and Flow Rates with fused core columns 5 Replies

Started by Tim Bannerjee in HPLC Posts. Last reply by Merlin K. L. Bicking, Ph.D. 1 hour ago.

Mike

Mixed Mode HPLC Phases 5 Replies

Started by Mike in HPLC Posts. Last reply by Merlin K. L. Bicking, Ph.D. 1 hour ago.

Dadang Gatot Prasetyo

The Best HPLC 12 Replies

Started by Dadang Gatot Prasetyo in HPLC Posts. Last reply by Merlin K. L. Bicking, Ph.D. 1 hour ago.

Aleš Čerin

How good should be water for chromatography? 8 Replies

Started by Aleš Čerin in HPLC Posts. Last reply by Merlin K. L. Bicking, Ph.D. 2 hours ago.

Michael Goodrich

Peak Tailing Criteria 5 Replies

Started by Michael Goodrich in GC Posts. Last reply by Thomas Wardley Jul 26.

Fabien Corrihons

HILIC vs ANP mode chromatography 2 Replies

Started by Fabien Corrihons in HPLC Posts. Last reply by Tobias Jonsson Jul 26.

korcan korba

How to calculate the free column volume and flow rate for each column? 5 Replies

Started by korcan korba in GC Posts. Last reply by Rakesh Mendhe Jul 25.

Peter Houston

The CHROMmunity proudly presents... YOU! 38 Replies

Started by Peter Houston in Off Topic. Last reply by Rajesh Galgalikar Jul 24.

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Blog Posts

Thomas Wardley

How I knew I was a Chromatographer

Posted by Thomas Wardley on July 26, 2010 at 9:30am

Kevin A. Schug

It's exponential, not geometric

Posted by Kevin A. Schug on July 23, 2010 at 3:54am — 1 Comment

Jared Anderson

The death of Dr. Leslie Ettre

Posted by Jared Anderson on July 18, 2010 at 8:04pm — 4 Comments

Jiri Urban

History of monolithic stationary phases

Posted by Jiri Urban on March 23, 2010 at 10:00pm — 4 Comments

Douglas Kitson

Strange samples

Posted by Douglas Kitson on February 8, 2010 at 12:58pm — 1 Comment

Erik Naylor

Foul sample matrices and the HPLC

Posted by Erik Naylor on February 5, 2010 at 11:44pm

Janet Kelsey

Help on hand for C18 column selection

Posted by Janet Kelsey on February 4, 2010 at 2:48pm

Peter Houston

WANTED! CHROMmunity Bloggers

Posted by Peter Houston on January 21, 2010 at 2:54pm

Peter Houston

Happy New Year from all at the CHROMmunity

Posted by Peter Houston on January 7, 2010 at 2:00pm

 
 
 

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